Composite
Part:BBa_K180019
Designed by: Richard Wilson, Stephanie Chambers Group: iGEM09_Southampton (2009-10-19)
Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]
Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1825
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1825
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1825
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1825
Illegal NgoMIV site found at 2071 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2014
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
control | trp, lac |
direction | forward |
emission | 553 nm |
emit | 553 |
excitation | 540 nm |
excite | 540 |
lum | |
n/a | Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator] |
protein | mBanana |