Plasmid_Backbone

Part:BBa_K5531006

Designed by: XI ZHAO   Group: iGEM24_SubCat-Union   (2024-09-02)
Revision as of 03:11, 2 September 2024 by Registry (Talk | contribs)

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pET-Dual-N-His-TEV

pET-Dual-N-His-TEV


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 305
    Illegal BamHI site found at 119
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1256


[edit]
Categories
Parameters
None