Coding
Part:BBa_K5271003:Design
Designed by: Yin Yan Chan Group: iGEM24_HKPOLYU (2024-09-28)
HER2-binding peptide -scrambled
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our preliminary results when it is joined with the EGFR nanobody by a linker, the linker should avoid cysteine residues since it potentially reduces the solubility of the dual targeting nanobody in prokaryotic expression system.
Source
The sequence of the HER2-binding peptide -scrambled was randomly generated and had a same length with the EGFR binding region of Panobody.