Primer

Part:BBa_K5269003

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)
Revision as of 03:23, 29 September 2024 by Nakaiyuichiro (Talk | contribs)


Surface displayed protein forward primer 1

This is a forward primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for the closest tac promoter(BBa_K5269033)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None