Part:BBa_K180010

Rock-paper-scissors - Rock primary plasmid

Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.

Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 2760
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 893
  Illegal NheI site found at 916
  Illegal PstI site found at 2760
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 622
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 2760
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 2760
  Illegal AgeI site found at 3365
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 147