Primer

Part:BBa_K5269011

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)
Revision as of 06:28, 28 September 2024 by Registry (Talk | contribs)

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Golden Gate Assembly Site In-Fusion forward primer 1

This forward primer is used for Golden Gate Assembly Site (BBa_K5269020), together with Golden Gate Assembly Site In-Fusion reverse primer 2 (BBa_K5269012) for PCR and In-Fusion of Golden Gate Assembly Site (BBa_K5269020) to pBluescript II SK(-). Used for PCR and In-Fusion of Golden Gate Assembly Site (BBa_K5269020) to pBluescript II SK(-).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None