Composite

Part:BBa_K180014

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-21)
Revision as of 12:43, 21 October 2009 by Registry (Talk | contribs)

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Rock-paper-scissors - Paper primary plasmid

Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand.

Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 945
    Illegal NheI site found at 968
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3104


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
emission511 nm
emit511
excitation501 nm
excite501
lum
proteinGFPmut3b