Part:BBa_K5115064

hox and hyp, with EP targeted hoxU

Introduction

This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. It's worth mention that, in this part, the subunit hoxU is fused with EP, check BBa_K5115059(hoxU fused with EP)for more details. To learn more about the hydrogenase, please check BBa_K51150020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

Usage and biology

This part is crucial in BBa_K5115066(mineral, U module), click on the link to view the experiment details.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 654
  Illegal BglII site found at 1472
  Illegal BglII site found at 1765
  Illegal BglII site found at 10143
  Illegal BglII site found at 10221
  Illegal BamHI site found at 2059
  Illegal XhoI site found at 99
  Illegal XhoI site found at 2266
  Illegal XhoI site found at 4505
  Illegal XhoI site found at 6334
  Illegal XhoI site found at 7496
  Illegal XhoI site found at 9335
  Illegal XhoI site found at 10151
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 533
  Illegal NgoMIV site found at 1071
  Illegal NgoMIV site found at 1281
  Illegal NgoMIV site found at 1593
  Illegal NgoMIV site found at 2359
  Illegal NgoMIV site found at 3039
  Illegal NgoMIV site found at 5646
  Illegal AgeI site found at 6821
  Illegal AgeI site found at 10104
  Illegal AgeI site found at 11103
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4159
  Illegal BsaI site found at 9925
  Illegal BsaI site found at 10087
  Illegal BsaI.rc site found at 702
  Illegal BsaI.rc site found at 1206
  Illegal SapI.rc site found at 10036