Part:BBa_K5115042

ribozyme connected: MTA, RcnR_C35L, Hpn

Introduction

This composite part combines BBa_K51150035(ribozyme+RBS+MTA+stem-loop), BBa_K51150033(ribozyme+RBS+RcnR_C35L+stem-loop) and BBa_K51150036(ribozyme+RBS+hpn+stem-loop) in our ribozyme-assisted polycistronic co-expression system：pRAP. To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

MTA is a protein that can bind with nickel ions to reduce its toxity to the 'E.coli'. The hpn is a protein that can sequester metals that accumulate internally to reduce nickel's toxity to the 'E.coli'. RcnR_C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 642
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 198
  
• 1000
  COMPATIBLE WITH RFC[1000]