Reporter
mCherry

Part:BBa_K5490025

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 10:15, 25 September 2024 by Registry (Talk | contribs)

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mCherry NLS

An mCherry fluorescent protein is separately expressed and directed to the nucleus via a nuclear localization signal (NLS). The purpose of mCherry is to act as a reporter, enabling independent monitoring of the CasRx activity and target RNA levels. This separation of signals allows for precise tracking of the effector molecule (CasRx) and the concentration of the target RNA, enhancing the accuracy of the experiment.

By monitoring mCherry fluorescence in the nucleus, researchers can confirm the presence of CasRx without interference from the target RNA cleavage activity, ensuring clear and accurate results in gene silencing studies or antiviral applications.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None