Plasmid

Part:BBa_K5490036

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 23:37, 28 September 2024 by Tzonissss13 (Talk | contribs)


10x[AB]-pMIN-fL

Is a type of promoter with high specificity to a particular type of transcription factor, in this case, the synthetic NFAT (BBa_K5490017), it achieves this by having various upstream elements of a small DNA sequence that contains the TATA box where polymerase II will bind. "Downstream, it contains a luciferase gene for assessing promoter strength through a luciferase assay.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 5571
    Illegal XbaI site found at 1457
    Illegal XbaI site found at 3239
    Illegal SpeI site found at 5325
    Illegal PstI site found at 1451
    Illegal PstI site found at 4414
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 5571
    Illegal SpeI site found at 5325
    Illegal PstI site found at 1451
    Illegal PstI site found at 4414
    Illegal NotI site found at 4393
    Illegal NotI site found at 5717
    Illegal NotI site found at 5787
    Illegal NotI site found at 6082
    Illegal NotI site found at 6152
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 5571
    Illegal BamHI site found at 1719
    Illegal XhoI site found at 6339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 5571
    Illegal XbaI site found at 1457
    Illegal XbaI site found at 3239
    Illegal SpeI site found at 5325
    Illegal PstI site found at 1451
    Illegal PstI site found at 4414
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1270
    Illegal EcoRI site found at 5571
    Illegal XbaI site found at 1457
    Illegal XbaI site found at 3239
    Illegal SpeI site found at 5325
    Illegal PstI site found at 1451
    Illegal PstI site found at 4414
    Illegal NgoMIV site found at 1028
    Illegal NgoMIV site found at 1049
    Illegal NgoMIV site found at 1364
    Illegal NgoMIV site found at 1472
    Illegal NgoMIV site found at 2950
    Illegal NgoMIV site found at 2967
    Illegal NgoMIV site found at 3099
    Illegal AgeI site found at 752
    Illegal AgeI site found at 3245
    Illegal AgeI site found at 5583
    Illegal AgeI site found at 5923
    Illegal AgeI site found at 5948
    Illegal AgeI site found at 6288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1740
    Illegal SapI site found at 3451
    Illegal SapI.rc site found at 934


Meško M, Lebar T, Dekleva P, Jerala R, Benčina M. Engineering and Rewiring of a Calcium-Dependent Signaling Pathway. ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20. PMID: 32643923; PMCID: PMC7467823.

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