Plasmid_Backbone

Part:BBa_K5490034

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)
Revision as of 15:05, 25 September 2024 by Registry (Talk | contribs)

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pCMV-FLAG-TRIM32

Vector of BBa_K5490017


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4637
    Illegal SpeI site found at 3999
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4637
    Illegal SpeI site found at 3999
    Illegal NotI site found at 4629
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4637
    Illegal BglII site found at 4649
    Illegal BamHI site found at 1
    Illegal XhoI site found at 995
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4637
    Illegal SpeI site found at 3999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4637
    Illegal SpeI site found at 3999
    Illegal NgoMIV site found at 3337
    Illegal AgeI site found at 553
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 391
    Illegal BsaI.rc site found at 2360
    Illegal SapI site found at 1277


[edit]
Categories
Parameters
None