Part:BBa_K4673024
The repressor binds to the operator
Within many prokaryotic bacterial structures, the operator serves as the primary region of DNA where the regulatory molecules of an operon are attached. The operator doesn’t exist in eukaryotes. Instead, it is the transcription factors that control the interactions associated with promoter portions.
In E. coli strains, the lac operator segment is generally characterized as a portion situated between the lac operon and the lac promoter, and it interacts with the repressor molecule(lacI). On DNA sequences, the lac repressor(lacI) specifically binds to the lac operator, inhibiting the expression of the lac operon. The binding also prevents the RNA polymerase from initiating transcription for the genes situated downstream of the lac operator since the lac operator overlaps the lac promoter. Consequently, the primary job of the lac operator is to regulate the process of gene expression by facilitating the binding of the operon and the regulatory molecules.
As a part of the pET-22b-LK vector in our team’s project, the lac operator functions as the binding region for the lacI repressor, playing an important role in the IPTG induction mechanism. The expression of lac operon occurs only when lactose is present and glucose is absent. The structure of the lac operon comprises the lac operator, which is a negative regulatory overlap with the promoter. Therefore, when lac repressor binds to lac operator, RNA polymerase cannot effectively attach to the promoter and start transcription.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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