RNA

Part:BBa_K5078002

Designed by: Jonathan Kinross   Group: iGEM24_UVU-Utah-2   (2024-08-07)
Revision as of 19:47, 28 September 2024 by Edomyan (Talk | contribs)


PTC amiRNA

An aritificial micro RNA used to silence the expression of the Phosphate Transport Channel 1 (PTC1) gene in Chlamydomonas reinhardtii thus removing the PTC1 transport protein from the central vacuole of the cell and preventing leakage of phosphate back into the environment in which our modified C. reinhardtii cell is placed.


Figure 1. Ptc miRNA in a level 0 plasmid for later golden gate assembly.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 85
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None