Plasmid_Backbone

Part:BBa_K5143005:Design

Designed by: Jess Philippon   Group: iGEM24_UnivLyon1-INSALyon   (2024-07-29)
Revision as of 11:39, 29 July 2024 by Registry (Talk | contribs)

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backbone for integrating genetic elements into the Saccharomyces cerevisiae genome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3973
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
    Illegal XhoI site found at 1605
    Illegal XhoI site found at 3446
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 3440
    Illegal EcoRI site found at 3967
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3440
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 714


Design Notes

test


Source

test

References