Regulatory
pLacIQ1

Part:BBa_K091112:Experience

Designed by: Pallavi Penumetcha   Group: iGEM08_Davidson-Missouri_Western   (2008-06-20)
Revision as of 21:52, 20 July 2009 by Alyndria.thompson (Talk | contribs) (→‎User Reviews)

This part worked as expected in HB101 cells.
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Applications of BBa_K091112

User Reviews

The MWSU/Davdison iGEM 2009 team sequenced this promoter after obtaining gel verification results indicating that the part was too large. The team found that there was a 35 bp insertion within this promoter directly before the suffix. We believe that the E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and select against an attribute that did not necessarily improve its fitness. Here is the 35 bp insertion:

TGTGTGGAATTGTGAGCGGATAACAATTTCACACA


UNIQ300a4cb452af5018-partinfo-00000000-QINU UNIQ300a4cb452af5018-partinfo-00000001-QINU