Part:BBa_K5246017

HB HfsE glycosyltransferase

Usage and Biology

The HfsE gene encodes a 518 amino acid protein in Hirschia baltica, identified as a putative integral membrane glycotransferase. The primary role of this protein is to transfer a glucose-1-phosphate from uridine diphosphate glucose (UDP-glucose) or to an undecaprenyl phosphate (UP), which is a crucial lipid carrier that facilitates the transfer of cell wall intermediates across the cytoplasmic membrane in bacteria. This reaction represents the first step in the synthesis of the holdfast polysaccharide repeat units in the cytoplasm. [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 48
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 214
  Illegal AgeI site found at 1381
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Chepkwony, N.K., Hardy, G.G. and Brun, Y.V. (2022a) ‘HFAE is a component of the holdfast anchor complex that tethers the holdfast adhesin to the cell envelope’, Journal of Bacteriology, 204(11). doi:10.1128/jb.00273-22.
2. Toh, E., Kurtz, H.D. and Brun, Y.V. (2008a) ‘Characterization of the caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in the initiating glycosyltransferase and polymerase steps’, Journal of Bacteriology, 190(21), pp. 7219–7231. doi:10.1128/jb.01003-08.