Coding

Part:BBa_K5258001

Designed by: XINYI CHEN   Group: iGEM24_ULC   (2024-08-01)
Revision as of 06:37, 1 August 2024 by Registry (Talk | contribs)

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25#SBD

A typical SBD is monomeric, composed of ~160 amino acids, and contains a hydrophobic surface cavity. The SBD recognizes PT-DNA by embedding sulfur into its hydrophobic cavity, hydrogen bonds, and electrostatic interactions between the SBD and the DNA, significantly contributing to binding. SBD proteins have a binding solid affinity on PT-DNA, primarily when binding to PT-double-strand (ds)DNA. This allows SBD as a targeting tool in which synthetic PT-modified oligos were used as probes to anneal with target single-strand DNA and form a hemi PT-modified double strand to be recognized by SBD.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None