Composite

Part:BBa_K5199004

Designed by: Hannah Grover   Group: iGEM24_USAFA   (2024-09-27)
Revision as of 22:45, 27 September 2024 by Registry (Talk | contribs)

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INP Silicatein

Ice Nucleation Protein(INP)- Silicatein Fusion Protein

INP is a transmembrane protein that acts as an external display marker. INP fusion proteins can be successfully expressed on the outside of E. coli. INP has been used by other teams (K409000 and K4578009), here we combined INP with a truncated silicatein alpha gene which polymerizes and precipitate silica. With external silicatein expression, we are able to precipitate silica on the outside of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 321
    Illegal XhoI site found at 1246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 396
    Illegal AgeI site found at 498
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 765
    Illegal SapI.rc site found at 229


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Categories
Parameters
None