Part:BBa_K5246011

CB2/CB2A HfsK Acetyltransferase

Usage and Biology

HfsK gene from Caulobacter crescentus encodes an acetyltransferase protein of 359aa. HfsK is c-di-GMP effector involved in holdfast biogenesis. Cells lacking HfsK form highly malleable holdfast structures with reduced adhesive strength that cannot support surface colonization. HfsK is a soluble protein but associates with the cell membrane during most of the cell cycle but is transfered to cytosol in the process of holdfast synthesis. HfsK deacetylates N-acetyl-glucosamine from the holfast which results in better adhesive properties.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 64
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 64
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 793
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 64
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 64
  
• 1000
  COMPATIBLE WITH RFC[1000]