DNA

Part:BBa_K5115083

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 16:52, 27 September 2024 by Shiyi2003 (Talk | contribs) (→‎Agarose gel electrophoresis)


deleted083

contributed by Fudan iGEM 2023

Introduction

Usage and Biology

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2024
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

M: DNA Marker; From left lane(1) to right lane(5) indicate the successful construction of RcnA KO doner in the plasmid of pJOE_pvdJ https://www.addgene.org/110142/

Successful Protein Expression

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

contributed by Fudan iGEM 2023
[edit]
Categories
Parameters
None