Part:BBa_K5109021

This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with LacE (BBa_K5109014). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.

This surface display system aims to express the laccase on the outer side of the cell membrane. This way, we could test it's functioning on PFAS molecules directly on the external medium, without stressing or damaging E. coli by expressing the enzyme inside.