Plasmid

Part:BBa_K5143027

Designed by: Perrine Ferdinand   Group: iGEM24_UnivLyon1-INSALyon   (2024-09-27)
Revision as of 15:14, 27 September 2024 by Registry (Talk | contribs)

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pUC57-pGAP-AF_V1-Venus-ENO1_term-p_ADH1-AGA2-mRuby2-6xHis-TDH1_term

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 4360
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
    Illegal SpeI site found at 4361
    Illegal PstI site found at 4375
    Illegal NotI site found at 4368
    Illegal NotI site found at 8332
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
    Illegal XhoI site found at 5964
    Illegal XhoI site found at 7805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 4361
    Illegal EcoRI site found at 7799
    Illegal EcoRI site found at 8326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 8326
    Illegal EcoRI site found at 7799
    Illegal SpeI site found at 4361
    Illegal PstI site found at 4375
    Illegal NgoMIV site found at 2077
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2727
    Illegal SapI.rc site found at 5073


[edit]
Categories
Parameters
None