Composite

Part:BBa_K5366024

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)
Revision as of 14:22, 27 September 2024 by Registry (Talk | contribs)

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T7 promoter-RBS-TET-6xHis-T7 termonator

TET structural gene expression plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal NheI site found at 1564
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal BglII site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
    Illegal NgoMIV site found at 795
    Illegal AgeI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None