Coding

Part:BBa_K5246006

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-22)
Revision as of 12:42, 22 September 2024 by Zaboras26 (Talk | contribs)


CB2/CB2A HfsF Flippase

Introduction

Usage and Biology

Encodes an integral membrane protein of 480 aa with 14 predicted transmembrane helices. Posseses similarities to Wzx flippases (formerly known as RfbX) that catalyze the translocation of undecaprenol diphosphate-linked K-repeating units formed at the cytoplasmic side of the inner membrane across this membrane

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1033
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1033
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1033
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1033
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 451
    Illegal NgoMIV site found at 733
    Illegal NgoMIV site found at 790
    Illegal NgoMIV site found at 834
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 1089
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Experimental characterization

References

[edit]
Categories
Parameters
None