Part:BBa_K5184059

st-t76SlCPR2

To equip our insecticide with enhanced prevention efficacy against T. urticae, we also decide to synthesize 9-hydroxy-zingiberene (9HZ) and 9-hydroxy-10,11-epoxy zingiberene (9H10epoZ), two oxidized products of the monocyclic sesquiterpene 9epiZ. However, since the reductase SlCPR2 was originally found in eukaryotic organisms. They were originally immobalized on the ER membrane. However, since E. coli does not have this structure, the 76 amino acid N-terminus ER transit peptide of the reductase is truncated to enhance solubility and expression rate. Also, a SpyTag is added, which will form an isopeptide bond with the SpyCatcher, thus imitating the colocalization of the two enzymes in eukaryotes. Our usage of st-t76SlCPR2 provide future iGEM teams with a novel method to synthesize an enzyme originally found in eukaryotes through a prokaryotic chassis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]