Coding

Part:BBa_K5184042

Designed by: Qianyue Jin   Group: iGEM24_GreatBay-SCIE   (2024-09-15)
Revision as of 11:23, 15 September 2024 by Registry (Talk | contribs)

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PpVP1-S

PpVP1-S is the shorter version of a venom peptide from Phytoseiulus persimilis, a predatory mite mainly preys on T. urticae. In our project, this protein's production is aimed to produce a more potent and specific venom peptide against the red spider mite, T. urticae. The coding sequence of this venom peptide is identified by gene alignment of NbSVP1(Bka:xxxxx) against the P. persimilis genome (GenBank: GCA_037576195.1). With the exons filtered out, the coding sequence is identified. With structure prediction results from Alphafold Server, the protein is modifed from PpVP2-F via truncation of ()aa on N-terminus and ()aa on the C-terminus ti achieve higher expression rate and possibly better toxicity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None