Composite

Part:BBa_K5311018

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-27)
Revision as of 10:52, 27 September 2024 by Carlospdag (Talk | contribs)


MG_010 + RNA thermometer ForU + mCherry + His-tag

Genetic construct created to produce sfGFP to induce fluorescence in C. acnes. This expression is regulated with an RNA Thermometer that allows expression when the optimal temperature of 37°C is achieved

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 453
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 453
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None