Composite

Part:BBa_K5477023

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)
Revision as of 18:57, 26 September 2024 by Registry (Talk | contribs)

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pSTE12-AhR - receptor module

The pSTE12-AhR receptor module is a composite designed to harness the natural ligand-sensing ability of the Aryl Hydrocarbon Receptor (AhR), integrated with the yeast promoter pSTE12. In this module, AhR is expressed under the control of the pSTE12 promoter. AhR is a ligand-activated transcription factor that binds to a variety of environmental contaminants, including polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and dioxins. Upon binding these ligands, AhR translocates from the cytoplasm to the nucleus, where it forms a heterodimer with the Aryl hydrocarbon receptor nuclear translocator (ARNT). This complex then binds to xenobiotic response elements (XREs), initiating the transcription of detoxification genes, such as those encoding cytochrome P450 enzymes.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 927
    Illegal XbaI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 927
    Illegal NheI site found at 732
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 927
    Illegal BglII site found at 1742
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 927
    Illegal XbaI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 927
    Illegal XbaI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None