Composite

Part:BBa_K5396011

Designed by: Alex Johan Mendes Comodaro   Group: iGEM24_CNPEM-BRAZIL   (2024-09-06)
Revision as of 11:46, 20 September 2024 by Jocomodaro (Talk | contribs)


T7-Nt-Barbie1-Cys

This composite part codes for the N terminal of Nt2RepCt fused with BARBIE1 protein (BBa_K53960010).

This part consists of the following basic parts: BBa_J435350, BBa_J435345, BBa_K53960010 and BBa_J428069.

Part Generation

Gibson Assembly

Expression and purification

Testing

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal AgeI site found at 743
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None