Coding
Part:BBa_K5115050
Designed by: Yi Shi Group: iGEM24_Fudan (2024-09-19)
MTA, Metallothionein
Contents
Introduction
Metallothioneins (MTA) are intracellular, low molecular, low molecular weight, cysteine-rich proteins. Ubiquitous in eukaryotes, MTA have unique structural characteristics to give potent metal-binding and redox capabilities.[1] Closely binding with nickel ion, MTA can reduce the harm this metal may cause to the bacteria.
Usage and Biology
In our design, MTA mainly works with BBa_K5115000(RcnR_C35L)and BBa_K1151001(hpn). They can raise the absorptivity of nickel ion as well as reduce the harm nickel brings to our engineering bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 123
- 1000COMPATIBLE WITH RFC[1000]
- ↑ Coyle, P., Philcox, J. C., Carey, L. C., & Rofe, A. M. (2002). Metallothionein: The multipurpose protein. Cellular and Molecular Life Sciences: CMLS, 59(4), 627–647.
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Categories
Parameters
| None |