Composite
hok/sok

Part:BBa_K5291037:Design

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-09-27)
Revision as of 13:47, 26 September 2024 by Registry (Talk | contribs)

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pAB-hok/sok


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The hok/sok system and Bi tern are from Escherichia coli but we have found that they are effective in engineered Pseudomonas aeruginosa. And we use promoters PcW and PopdH native in P. aeruginosa to ensure the effect of gene expression.


Source

hok/mok, sok, Bi tern: Escherichia coli PcW, PopdH: Pseudomonas aeruginosa PAO1

References