Device

Part:BBa_K5152008

Designed by: Tian Xin Ho   Group: iGEM24_HongKong-JSS   (2024-09-24)
Revision as of 07:00, 26 September 2024 by Pok430 (Talk | contribs)


MerR-pMerT mercury sensing chromoprotein reporter device

This device contains the mercury-inducible promoter pMerT and co-expresses its repressor protein MerR, which specifically binds to mercury. When mercury is present, the pMerT promoter drives the expression of the chromoprotein tsPurple, serving as the reporter signal. This device is specifically designed to detect the presence of mercury in the environment.

However, because we ordered the wrong DNA, we didn't have time to clone this construct, and no experiments were conducted on it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None