Part:BBa_K5477005
pGAL1/10 - bidirectional inducible promoters from Saccharomyces cerevisiae
The GAL1 and GAL10 promoters are two tightly linked, yet functionally distinct, promoters found in Saccharomyces cerevisiae. They regulate the expression of genes involved in galactose metabolism. The GAL1 promoter drives the expression of the GAL1 gene, which encodes galactokinase, an enzyme responsible for the phosphorylation of galactose. The GAL10 promoter controls the GAL10 gene, encoding UDP-glucose-4-epimerase, another key enzyme in the galactose metabolic pathway (1). Both promoters are induced in the presence of galactose and repressed by glucose through the action of regulatory proteins such as Gal4p and Gal80p. The upstream activating sequences (UAS) located between the two promoters are critical for the activation of both promoters, and Gal4p binds these UAS elements to initiate transcription when galactose is present.
The GAL1/10 bidirectional promoter was formed by combining these two promoters, with the UAS elements located centrally, allowing for the simultaneous regulation of both genes in opposite directions.
pGAL1/10 was used to drive the expression of our detoxification system. The following composite parts were built:
UDPD-pGAL1/10-UGT2B15 UDPD-pGAL1/10-UGT1A1 CYP1A1-pGAL1/10-POR CYP3A4-pGAL1/10-POR CYP3A4-MYC-pGAL1/10-POR
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 374
- 1000COMPATIBLE WITH RFC[1000]
References
1. West RW Jr, Chen SM, Putz H, Butler G, Banerjee M. GAL1-GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequences. Genes Dev. 1987 Dec;1(10):1118-31. doi: 10.1101/gad.1.10.1118. PMID: 3322938.
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