Regulatory

Part:BBa_K5477002

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-25)
Revision as of 19:28, 25 September 2024 by Kateesc1700 (Talk | contribs)


pRAD27 - weak constitutive promoter in Saccharomyces cerevisiae

RAD27 encodes a 5' to 3' exonuclease and 5' flap endonuclease, which is essential for Okazaki fragment processing and maturation during DNA replication. It plays a role in long-patch base-excision repair, large loop repair (LLR), and ribonucleotide excision repair. RAD27 removes RNA flaps in RNA:DNA hybrid intermediates during lagging strand synthesis. It can also relocalize to the cytosol under hypoxic conditions (1) (2) (3) (4) (6) (7).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Ayyagari R, Gomes XV, Gordenin DA, Burgers PM. Okazaki fragment maturation in yeast. I. Distribution of functions between FEN1 AND DNA2. J Biol Chem. 2003 Jan 17;278(3):1618-25. doi: 10.1074/jbc.M209801200. Epub 2002 Nov 6. PMID: 12424238.

2. Dastidar RG, Hooda J, Shah A, Cao TM, Henke RM, Zhang L. The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci. 2012 Aug 29;2(1):30. doi: 10.1186/2045-3701-2-30. PMID: 22932476; PMCID: PMC3489556.

3. Kao HI, Henricksen LA, Liu Y, Bambara RA. Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate. J Biol Chem. 2002 Apr 26;277(17):14379-89. doi: 10.1074/jbc.M110662200. Epub 2002 Feb 1. PMID: 11825897.

4. Laverde EE, Polyzos AA, Tsegay PP, Shaver M, Hutcheson JD, Balakrishnan L, McMurray CT, Liu Y. Flap Endonuclease 1 Endonucleolytically Processes RNA to Resolve R-Loops through DNA Base Excision Repair. Genes (Basel). 2022 Dec 29;14(1):98. doi: 10.3390/genes14010098. PMID: 36672839; PMCID: PMC9859040.

5. Lee, Michael & DeLoache, William & Cervantes, Bernardo & Dueber, John. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS synthetic biology. 4. 10.1021/sb500366v.

6. Sommer D, Stith CM, Burgers PM, Lahue RS. Partial reconstitution of DNA large loop repair with purified proteins from Saccharomyces cerevisiae. Nucleic Acids Res. 2008 Aug;36(14):4699-707. doi: 10.1093/nar/gkn446. Epub 2008 Jul 15. PMID: 18628298; PMCID: PMC2504288.

7. Sparks JL, Chon H, Cerritelli SM, Kunkel TA, Johansson E, Crouch RJ, Burgers PM. RNase H2-initiated ribonucleotide excision repair. Mol Cell. 2012 Sep 28;47(6):980-6. doi: 10.1016/j.molcel.2012.06.035. Epub 2012 Aug 2. PMID: 22864116; PMCID: PMC3470915.

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