Part:BBa_K5143001
MaSp1 : a spider silk protein used as a bioglue, optimised for Saccharomyces cerevisiae
Description
MaSp1 is a spider silk protein that can be used as a bioglue. The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m².
Construction
The MaSp1 gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a barnacle ciment protein as a bioglue, to improve its adhesive properties: BBa_K5143003
References
Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 22
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 1000COMPATIBLE WITH RFC[1000]
| None |