Device

Part:BBa_K195622

Designed by: Yun-Hsiang Chang   Group: iGEM09_NYMU-Taipei   (2009-10-18)
Revision as of 17:07, 21 October 2009 by Benjamin (Talk | contribs)

CII/TetR Damped Oscillator With GFP Reporter (I)

This device is produces damped oscillations between TetR and CII. The pRE (BBa_K116603) can be activated by CII (BBa_K116602), which in turn produces TetR (BBa_C0040) and GFP. The TetR then represses the pTet promoter (BBa_R0040) resulting in slower production of CII.

This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.

Modeling Data

Er3tAr2tEr7t.png

  • Figure 1. This Graph is the modeling of our CII/TetR damped oscillator. There are more details on 2009 NYMU-Taipei iGEM team Wiki page.
    • [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal/Modeling Modeling data]
    • [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal#Graph_of_Experiments Experiment data]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2141


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Categories
Parameters
None