Reporter

Part:BBa_I13600:Experience

Designed by: Christopher Batten, Victoria Chou, Kenneth Nesmith   Group: Antiquity   (2004-07-16)
Revision as of 17:10, 21 October 2009 by Dan (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I13600

  • Grew up this Biobrick on a plate just to test its flourescence compared to the other constitutive flourescent proteins in the Registry as of July 2006. This has one of the weakest flourescent outputs. --Smelissali 19:39, 3 July 2006 (EDT)

User Reviews

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Smelissali

Brick works, but the cyan flourescent protein is definitely not the brightest reporter you could use, and thus not my first choice. --Smelissali 19:38, 3 July 2006 (EDT)

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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Aberdeen_Scotland 2009

The gel analysis and the plasmid miniprep worked quite nicely as expected. But later the construct was further tested and characterised by combining with the bio-brick BBa_K182002 which contained a tet repressor under the control of a CI operator. So, the CFP expression of BBa_I13600was repressed as expected when tested with the BBa_K182002. With the addition of Anhydrotetracycline (an analogue of tetracycline) the CFP expression was restored and this was confirmed performing the fluorescent microscopy assay. Expression can be visualised very nicely under a flurescent microscope. Even without having ideal filter. We used GFP filter for the picture below. [[Image:2 I13600 flu60aberdeen2009.jpg|center|400 px]]

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