Reporter
Part:BBa_K191005
Designed by: Le Thanh Tu NGUYEN Group: iGEM09_EPF-Lausanne (2009-10-11)
TRP promoter - RBS - TetR - Term - TetR promoter - RBS - RFP - Term
TRP inhibits TetR's transcription while TetR inhibits RFP's transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 43
Illegal SpeI site found at 51 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 43
Illegal SpeI site found at 51
Illegal AgeI site found at 1576
Illegal AgeI site found at 1688 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//function/reporter/fluorescence
emission | 630nm |
input_s | Trp, LovTAP, ATc |
positive_regulators | |
protein_out | RFP |