Part:BBa_K5186002
sRNA2(IspH)
Description
sRNA(IspH)s are synthetic small regulatory RNAs (sRNAs) designed to specifically down-regulate the expression of the IspH gene in E. coli, thereby enhancing HMBPP production for mosquito attraction. Each sRNA(IspH) is composed of a target-binding sequence that is complementary to the IspH mRNA, enabling hybridization, and a SgrS scaffold that facilitates the recruitment of the Hfq protein in E. coli. This interaction promotes the sRNAs' binding to ispH mRNA and expedites its degradation by RNase E. (Seung Min Yoo et al.) It has been revealed that the mutation in the SgrS scaffold can enhance the efficiency of sRNA-mediated gene repression (Minho Noh et al.). This year, we have successfully engineered 4 variants of sRNA(IspH)s in E. coli, each with a specialized scaffold. We expect to select the best one for controlling the down-regulation of IspH from them. And sRNA2(IspH) introduces a 6-nts stem with the SgrS-S scaffold, exhibiting the most excellent downregulation effect without affecting bacterial growth. These sRNA(IspH)s (BBa_K5186001, BBa_K5186002, BBa_K5186003, BBa_K5186004, BBa_K5186005) for the downregulation of downstream gene expression and various MEP overexpression cassettes (BBa_K5186006, BBa_K5186007, BBa_K5186008, BBa_K5186009) contribute to the overproduction of HMBPP in E. coli and thus make up a part collection. This collection can help and inspire other iGEM teams and researchers to achieve higher yield of HMBPP or other isoprenoids in E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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