Coding

Part:BBa_K2619004

Designed by: shiyu liu   Group: iGEM18_XJTLU-CHINA   (2018-09-08)
Revision as of 13:48, 8 September 2018 by Registry (Talk | contribs)

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19kDa nano luciferase/nanoLuc

this Nano lucierase (nluc) is the mutated catalytic 19 kDa component of Oplophorus luciferase. nluc is a newly developed luciferase. In our project, it can be expressed as an indicator of exosome quantity by linking it with CD63 and lamp2b. We use it as reporter of membrane protein gene expression. It is also combined with C/D box and 3 stem loop to exam the transportation efficiency of packaging devices.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None