Part:BBa_K5115010

hypF

Introduction

The hypF is a subunit of the hox and hyp operonBBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which ruduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115019(ribozyme+RBS+hypF+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiments.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 424
  Illegal XhoI site found at 2263
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 29
  Illegal NgoMIV site found at 250
  Illegal NgoMIV site found at 274
  Illegal NgoMIV site found at 635
  Illegal NgoMIV site found at 2035
  Illegal NgoMIV site found at 2435
  
• 1000
  COMPATIBLE WITH RFC[1000]