DNA
PopdH

Part:BBa_K5291021

Designed by: Rui Chen   Group: iGEM24_BNUZH-China   (2024-08-11)
Revision as of 02:30, 11 August 2024 by Registry (Talk | contribs)

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PopdH

This promoter comes from opdH-tctCBA-tctDE manipulator system in Pseudomonas aeruginosa and we name it PopdH. The tctD protein can specifically bind to PopdH, repressing its activity. While citrate exists in the peripheral environment, tctD tends to bind with citrate instead of PopdH, and the released PopdH is able to turn on the expression of genes downstream. This is what makes PopdH a promoter that is controlled by the concentration of citrate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None