Composite

Part:BBa_K195619

Designed by: Yun-Hsiang Chang   Group: iGEM09_NYMU-Taipei   (2009-10-18)
Revision as of 16:49, 21 October 2009 by Benjamin (Talk | contribs)

pLacI with activator gene CII and pRE with GFP generator

This part is composed of pLac with activator gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The pLac is repressed by LacI, which could be inhibited by IPTG.The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pLac and produce green fluorescent protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1383


[edit]
Categories
Parameters
None