Translational_Unit

Part:BBa_K216017:Design

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-21)
Revision as of 16:49, 21 October 2009 by Cfrench (Talk | contribs) (→‎References)

luxCDE genes of Xenorhabdus luminescens (aldehyde biosynthesis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1332


Design Notes

The normal operon structure is luxCDABE. This part is derived from an earlier (non-BioBrick) project, in which the luxCD genes were fused to luxE so that the aldehyde biosynthesis machinery could be induced separately from the luciferase. An XbaI site must be removed from within luxD.


Source

As part of an earlier project, the luxCD and luxE regions were separately amplified from genomic DNA of Xenorhabdus luminescens Hb (NCIMB 12670) and joined at a NotI site to create an artificially reduced luxCDE operon. This was tested and was found to work well in E. coli. The part shown here was made by amplifying this using primers which added the BioBrick prefix and suffix.

References

  • Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound. Journal of Bacteriology 173, 1399-1405.
  • Meighen, E.A., and Szittner, R.B. 1992. Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria. J. Bacteriol. 174, 5371-5381.