Part:BBa_K5115027

csoS3

Introduction

CsoSCA is a critical component of the carboxysome in cyanobacteria, specifically identified in Cyanobium sp. PCC7001. It plays a vital role in carbon concentrating mechanisms by enhancing CO2 fixation through its catalytic activity as a carbonic anhydrase (CA). Recent studies reveal that CsoSCA is allosterically activated by ribulose-1,5-bisphosphate (RuBP), underscoring its regulatory significance in photosynthesis.

Usage and Biology

CsoSCA's functionality is tightly linked to the presence of RuBP, which is essential for its enzymatic activity. It forms a hexameric structure that allows efficient CO2 hydration, enhancing the overall effectiveness of the carboxysome. Understanding the regulation and structure of CsoSCA is crucial for advancing knowledge in cyanobacterial biology and improving carbon fixation strategies in biotechnology applications.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 146
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Pulsford, S. B., Outram, M. A., Förster, B., Rhodes, T., Williams, S. J., Badger, M. R., Price, G. D., Jackson, C. J., & Long, B. M. (2024). Cyanobacterial α-carboxysome carbonic anhydrase is allosterically regulated by the Rubisco substrate RuBP. Science advances, 10(19), eadk7283. https://doi.org/10.1126/sciadv.adk7283