Composite

Part:BBa_K5057009

Designed by: Jan Zielinski   Group: iGEM24_Freiburg   (2024-09-23)
Revision as of 19:09, 23 September 2024 by Registry (Talk | contribs)

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mCherry with the extracellular signal peptide HSTII


mCherry is a very rapidly-maturing monomeric red fluorescent protein with low acid sensitivity.[1]. The DNA sequence of this biobrick encodes a version of mCherry with an additional sequence designed for liquid-liquid phase separation purposes and an extracellular localization sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 780
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 798


[edit]
Categories
Parameters
None