Translational_Unit
Part:BBa_K216008:Design
Designed by: Edinburgh iGEM 2009 Group: iGEM09_Edinburgh (2009-10-16)
bacterial luciferase LuxAB of Xenorhabdus luminescens
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 530
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1049
Design Notes
No special considerations. Please note that preliminary sequencing suggests that there may be a problem with the SpeI site of the construct submitted. If this is confirmed, we will submit a revised version and remove this note.
Source
Amplified from genomic DNA of Xenorhabdus luminescens strain Hb, obtained from the National Collection of Industrial and Marine Bacteria, Aberdeen, UK, strain NCIMB 12670.
References
- Xi, L., Cho, K.W., and Tu, S.C. 1991. Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound. Journal of Bacteriology 173, 1399-1405.
- Meighen, E.A., and Szittner, R.B. 1992. Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria. J. Bacteriol. 174, 5371-5381.
- Valkova, N., Szittner, R., and Meighen, E.A. 1999. Control of luminescence decay and flavin binding by the LuxA carboxy-terminal regions in chimeric bacterial luciferases. Biochemistry 38, 13820-13828.
- Westerlund-Karlsson, A., Saviranta, P., and Karp, M. 2002. Generation of thermostable monomeric luciferases from Photorhabdus luminescens. Biochemical and Biophysical Research Communications 296, 1072-1076.
- Mitchell, R.J., Ahn, J.M., and Gu, M.B. 2005. Comparison of Photorhabdus luminescens and Vibrio fischeri lux fusions to study gene expression patterns. Journal of Microbiology and Biotechnology 15, 48-54.