Composite

Part:BBa_K5036050

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-21)
Revision as of 15:28, 21 September 2024 by Registry (Talk | contribs)

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MMP9 Nanobody3- NSP3A

Our engineered switch contains NSP3 protein, which is linked to a nanobody that specifically recognizes MMP9. MMP9 is an enzyme that is elevated inside cells during injury. When injury occurs, the increased MMP9 binds to the nanobody, triggering a change in the switch's structure. This structural change causes a specific mRNA molecule to be transformed into a circular form, which promotes its translation into YAP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None