Other
Part:BBa_K5107000:Design
Designed by: Georgios Retsinias Group: iGEM24_DTU-Denmark (2024-09-22)
HRE_minimal
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter).
Source
The part is synthesised as g-block part of the the whole device we used in our cell free system by IDT.